Over a century ago, Miller wrote that bacteria are connected in some manner with pulpal diseases, and he raised the hypothesis that they are the causative factor for diseases of endodontic origin. This hypothesis has been proved as bacteria have been implicated in pathogenesis and progression of pulpal and periradicular diseases. Bacterial composition of the infected root canal is complex, mainly consisting of obligate anaerobes and facultative aerobes. The primary aim of endodontic treatment is to remove as many bacteria as possible from the root canal system and then create an environment in which the remaining microorganisms fail to thrive.
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